Background and purpose — Hypoxia, necrosis, and bone loss are hallmarks of many skeletal diseases. Hyperbaric oxygen therapy (HBO) is often used as an adjunctive therapy in these cases. However the in vivo effect of HBO on osteoclast formation has not been fully established. We therefore carried out a longitudinal study to examine the effect …
Objectives: This experimental study aimed to create a prefabricated vascularized bone graft using the interconnected porous coralline hydroxyapatite ceramic by combining vascular bundle implantation, mesenchymal stem cells, and hyperbaric oxygen therapy (HBOT) administration in a rat model. Methods: Forty-five male Sprague-Dawley rats were divided into three groups, each containing 15 rats. The hydroxyapatite ceramics were …
Effect of hyperbaric oxygen therapy on bone prefabrication in rats Read More »
In view of the controversy of the clinical use of hyperbaric oxygen (HBO) treatment to stimulate fracture healing and bone regeneration, we have analysed the effects of daily exposure to HBO on the proliferation and differentiation of human osteoblasts in vitro. HBO stimulated proliferation when osteoblasts were cultured in 10% foetal calf serum (FCS), whereas …
Abstract: Background: This study tested the hypothesis that hyperbaric oxygen (HBO) therapy enhanced the circulating levels of endothelial progenitor cells (EPCs), soluble angiogenesis factors, and blood flow in ischemic areas in patients with peripheral arterial occlusive disease(PAOD). Methods: In total, 57 consecutive patients with PAOD undergoing the HBO therapy (3 atmospheres (atm) for 2 h …
Significance: Oxidative stress is recognized as playing a role in stem cell mobilization from peripheral sites and also cell function. Recent Advances: This review focuses on the impact of hyperoxia on vasculogenic stem cells and elements of wound healing. Critical Issues: Components of the wound-healing process in which oxidative stress has a positive impact on …
Hyperbaric Oxygen, Vasculogenic Stem Cells, and Wound Healing Read More »
We hypothesized that oxidative stress from hyperbaric oxygen (HBO2, 2.8 ATA for 90 min daily) exerts a trophic effect on vasculogenic stem cells. Ina mouse model, circulating stem/progenitor cell (SPC) recruitment and differentiation in subcutaneous Matrigel were stimulated by HBO2 and by a physiological oxidative stressor, lactate. In combination, HBO2 and lactate had additive effects. …
Hyperbaric oxygen stimulates vasculogenic stem cell growth and differentiation in vivo Read More »
We hypothesized that tissue hyperoxia would enhance and hypoxia inhibit neovascularization in a wound model. Therefore, we used female Swiss-Webster mice to examine the influence of differential oxygen treat-ment on angiogenesis. One milliliter plugs of Matrigels, a mixture of matrix proteins that supports but does not itself elicit angiogenesis, were injected subcutaneously into the mice. …
Hyperoxia and angiogenesis Read More »
Hyperbaric oxygen (HBO) therapy has been used as an adjunctive therapy for diabetic foot ulcers, although its mechanism of action is not completely understood. Recently, it has been shown that HBO mobilizes the endothelial progenitor cells (EPCs) from bone marrow that eventually will aggregate in the wound. However, the gathering of the EPCs in diabetic …
Background:This paper outlines therapeutic mechanisms of hyperbaric oxygen therapy (HBO2) and reviews data on its efficacy for clinical problems seen by plastic and reconstructive surgeons.Methods: The information in this review was obtained from the peer-reviewed medical literature.Results: Principal mechanisms of HBO2 are based on intracellular generation of reactive species of oxygen and nitrogen. Reactive species …
Hyperbaric oxygen – its mechanisms and efficacy Read More »
THERAPEUTIC MECHANISMS OF action for hyperbaric oxygen(HBO2) are based on elevation of both the partial pressure ofoxygen and hydrostatic pressure. Elevating the hydrostaticpressure increases partial pressure of gases and causes a reductionin the volume of gas-filled spaces according to Boyle’slaw. These actions have direct relevance to treating pathologicalconditions in which gas bubbles are present in …
Oxidative stress is fundamental to hyperbaric oxygen therapy Read More »
